Expression of Mammalian Metallothionein Suppresses Glucosinolate Synthesis in Brassica campestris
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چکیده
منابع مشابه
Expression of Mammalian Metallothionein Suppresses Glucosinolate Synthesis in Brassica campestris.
Transfection of Brassica campestris leaves with cauliflower mosaic virus (CaMV) harboring a mammalian metallothionein (MT) cDNA at the ORFII position lowered the glucosinolate (GS) concentration to approximately one-half the level in leaves infected with wild-type CaMV. This suppression was independent of the plant's sulfate status, suggesting that the pathways for protein (MT) and GS biosynthe...
متن کاملModification of Leaf Glucosinolate Contents in Brassica oleracea by Divergent Selection and Effect on Expression of Genes Controlling Glucosinolate Pathway
Modification of the content of secondary metabolites opens the possibility of obtaining vegetables enriched in these compounds related to plant defense and human health. We report the first results of a divergent selection for glucosinolate (GSL) content of the three major GSL in leaves: sinigrin (SIN), glucoiberin (GIB), and glucobrassicin (GBS) in order to develop six kale genotypes (Brassica...
متن کاملIdentification and expression analysis of glucosinolate biosynthetic genes and estimation of glucosinolate contents in edible organs of Brassica oleracea subspecies.
Glucosinolates are anti-carcinogenic, anti-oxidative biochemical compounds that defend plants from insect and microbial attack. Glucosinolates are abundant in all cruciferous crops, including all vegetable and oilseed Brassica species. Here, we studied the expression of glucosinolate biosynthesis genes and determined glucosinolate contents in the edible organs of a total of 12 genotypes of Bras...
متن کاملSequence of a Brassica campestris myrosinase gene.
Myrosinase (thioglucoside glucohydrolase, P-thioglucosidase, EC 3.2.3.1) catalyzes the hydrolysis of glucosinolates, a diverse group of sulfur-containing glycosides that are present in a11 the Cruciferae, to D-G~c, sulfur, and a variety of volatile compounds including thiocyanates, isothiocyanates, and nitriles (Fenwick et al., 1983). Normally, myrosinase and the glucosinolates are kept sequest...
متن کاملExpression Profiling of Glucosinolate Biosynthetic Genes in Brassica oleracea L. var. capitata Inbred Lines Reveals Their Association with Glucosinolate Content.
Glucosinolates are the biochemical compounds that provide defense to plants against pathogens and herbivores. In this study, the relative expression level of 48 glucosinolate biosynthesis genes was explored in four morphologically-different cabbage inbred lines by qPCR analysis. The content of aliphatic and indolic glucosinolate molecules present in those cabbage lines was also estimated by HPL...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1990
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.93.2.522